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HiFi de novo genome assembly workflow

About

HiFi-assembly-workflow is a bioinformatics pipeline that can be used to analyse Pacbio CCS reads for de novo genome assembly using PacBio Circular Consensus Sequencing (CCS) reads. This workflow is implemented in Nextflow and has 3 major sections.

Please refer to this documentation for detailed recommendations relevant to each workflow section:

Documentation pages available

Please cite the workflow as follows

Contributors

Johan Gustafsson Documentation

Johan Gustafsson

Australian BioCommons / University of Melbourne

Kenneth Chan Workflow / documentation

Kenneth Chan

Australian Genome Research Facility

Martha Zakrzewski Workflow

Martha Zakrzewski

Australian Genome Research Facility

Naga Kasinadhuni Workflow / documentation

Naga Kasinadhuni

Australian Genome Research Facility

Uwe Winter Workflow

Uwe Winter

Australian BioCommons / University of Melbourne

Ziad Al Bkhetan Workflow / documentation

Ziad Al Bkhetan

Australian BioCommons / University of Melbourne

Acknowledgements

This work was developed at AGRF and supported by the Australian BioCommons via Bioplatforms Australia funding, the Australian Research Data Commons (https://doi.org/10.47486/PL105) and the Queensland Government RICF programme. Bioplatforms Australia and the Australian Research Data Commons are enabled by the National Collaborative Research Infrastructure Strategy (NCRIS).

The documentation in this repository is based on Australian BioCommons guidelines.

This guide makes use of the ELIXIR toolkit theme

Australian BioCommons
ELIXIR Toolkit Theme

References

Gustafsson, J., Al Bkhetan, Z., Shadbolt, M., Murigneux, V., Williams, S., Capon, P., Khan, F. Z., & Nelson, T. (2023). How-to Guide for creating a How-to Guide (Version 1.0.0) [Computer software]. https://doi.org/10.5281/zenodo.8210269