About
HiFi-assembly-workflow is a bioinformatics pipeline that can be used to analyse Pacbio CCS reads for de novo genome assembly using PacBio Circular Consensus Sequencing (CCS) reads. This workflow is implemented in Nextflow and has 3 major sections.
Please refer to this documentation for detailed recommendations relevant to each workflow section:
Documentation pages available
Please cite the workflow as follows
Contributors
Johan Gustafsson
Australian BioCommons / University of Melbourne
Kenneth Chan
Australian Genome Research Facility
Martha Zakrzewski
Australian Genome Research Facility
Naga Kasinadhuni
Australian Genome Research Facility
Uwe Winter
Australian BioCommons / University of Melbourne
Ziad Al Bkhetan
Australian BioCommons / University of Melbourne
Acknowledgements
This work was developed at AGRF and supported by the Australian BioCommons via Bioplatforms Australia funding, the Australian Research Data Commons (https://doi.org/10.47486/PL105) and the Queensland Government RICF programme. Bioplatforms Australia and the Australian Research Data Commons are enabled by the National Collaborative Research Infrastructure Strategy (NCRIS).
The documentation in this repository is based on Australian BioCommons guidelines.
This guide makes use of the ELIXIR toolkit theme
References
Gustafsson, J., Al Bkhetan, Z., Shadbolt, M., Murigneux, V., Williams, S., Capon, P., Khan, F. Z., & Nelson, T. (2023). How-to Guide for creating a How-to Guide (Version 1.0.0) [Computer software]. https://doi.org/10.5281/zenodo.8210269